References

farmaec

FARMAKOEKONOMIKA. Modern Pharmacoeconomics and Pharmacoepidemiology

ФАРМАКОЭКОНОМИКА. Современная фармакоэкономика и фармакоэпидемиология

2070-49092070-4933

IRBIS LLC

10.17749/2070-4909/farmakoekonomika.2021.114

farmaec-625

Research Article

ORIGINAL ARTICLES

ОРИГИНАЛЬНЫЕ ПУБЛИКАЦИИ

Peptides of Laennec® preparation that contribute to the elimination of endotheliopathy

Пептиды в составе препарата Лаеннек®, способствующие устранению эндотелиопатии

https://orcid.org/0000-0002-2659-7998

Торшин

И. Ю.

Torshin

I. Yu.

Торшин Иван Юрьевич – к.ф-м.н., к.х.н., старший научный сотрудник

WoS ResearcherID: C-7683-2018

Scopus Author ID: 7003300274

РИНЦ SPIN-код: 1375-1114

ул. Вавилова, д. 4, Москва 119333

Ivan Yu. Torshin – PhD (Phys. Math.), PhD (Chem.), Senior Researcher

Wos ResearcherID: C-7683-2018

Scopus Author ID: 7003300274

RSCI SPIN-code: 1375-1114

4 Vavilov Str., Moscow 119333

https://orcid.org/0000-0002-7663-710X

Громова

О. А.

Gromova

О. А.

Громова Ольга Алексеевна – д.м.н., профессор, научный руководитель

WoS ResearcherID: J-4946-2017

Scopus Author ID: 7003589812

РИНЦ SPIN-код: 6317-9833

ул. Вавилова, д. 4, Москва 119333

Olga A. Gromova – Dr. Med. Sc., Professor, Research Supervisor

Wos ResearcherID: J-4946-2017

Scopus Author ID: 7003589812

RSCI SPIN-code: 6317-9833

4 Vavilov Str., Moscow 119333

unesco.gromova@gmail.com

https://orcid.org/0000-0002-4532-4274

Згода

В. Г.

Zgoda

V. G.

Згода Виктор Гаврилович – д.б.н.

WoS ResearcherID: F-1791-2017

Scopus Author ID: 6602917155

РИНЦ SPIN-код: 7840-1330

ул. Погодинская, д. 10, стр. 8, Москва 119121

Viktor G. Zgoda – Dr. Biol. Sc.

WoS ResearcherID: F-1791-2017

Scopus Author ID: 6602917155

RSCI SPIN-code: 7840-1330

10 bld. 8. Pogodinskaya Str., Moscow 119121

https://orcid.org/0000-0002-5070-5450

Чучалин

А. Г.

Chuchalin

А. G.

Чучалин Александр Григорьевич – д.м.н., профессор, академик РАН, пульмонолог, заведующий кафедрой госпитальной терапии педиатрического факультета

РИНЦ SPIN-код: 7742-2054

ул. 1-я Леонова, д. 16, Москва 129226

Aleksandr G. Chuchalin – Dr. Med. Sc., Professor, Academician of RAS, Pulmonologist, Head of Chair of Hospital Therapy, Faculty of Pediatrics

RSCI SPIN-code: 7742-2054

16 Pervaya Leonov Str., Moscow 129226

https://orcid.org/0000-0003-4120-1071

Максимов

В. А.

Maksimov

V. А.

Максимов Валерий Алексеевич – д.м.н., профессор кафедры диетологии и нутрициологии

Scopus Author ID: 55901011200

ул. Баррикадная, д. 2, стр. 1, Москва 123995

Valeriy А. Maksimov – Dr. Med. Sc., Professor, Chair of Dietetics and Nutritionology

Scopus Author ID: 55901011200

2 bld. 1 Barrikadnaya Str., Moscow 123995

https://orcid.org/0000-0002-2810-566X

Тихонова

О. В.

Tikhonova

О. V.

Тихонова Ольга Валентиновна – к.б.н.

WoS ResearcherID: F-5115-2017

Scopus Author ID: 57189102916

РИНЦ SPINкод: 8320-9820

ул. Погодинская, д. 10, стр. 8, Москва 119121

Olga V. Tikhonova – PhD (Biol.)

WoS ResearcherID: F-5115-2017

Scopus Author ID: 57189102916

RSCI SPIN-code

10 bld. 8. Pogodinskaya Str., Moscow 119121

Федеральный исследовательский центр «Информатика и управление» Российской академии наукРоссияFederal Research Center “Informatics and Management”, Russian Academy of SciencesRussian Federation

Федеральное государственное бюджетное научное учреждение «Научно-исследовательский институт биомедицинской химии им. В.Н. Ореховича», Центр коллективного пользования «Протеом человека»РоссияOrekhovich Research Institute of Biomedical Chemistry, Center for Collective Use “Human Proteome”Russian Federation

Федеральное государственное автономное образовательное учреждение высшего образования «Российский национальный исследовательский медицинский университет им. Н.И. Пирогова» Министерства здравоохранения Российской ФедерацииРоссияPirogov Russian National Research Medical UniversityRussian Federation

Федеральное государственное бюджетное образовательное учреждение дополнительного профессионального образования «Российская медицинская академия непрерывного профессионального образования» Министерства здравоохранения Российской ФедерацииРоссияRussian Medical Academy of Continuing Professional EducationRussian Federation

2021

15012022

144468479

2022

Торшин И.Ю., Громова О.А., Згода В.Г., Чучалин А.Г., Максимов В.А., Тихонова О.В.

Torshin I.Y., Gromova О.А., Zgoda V.G., Chuchalin А.G., Maksimov V.А., Tikhonova О.V.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://www.pharmacoeconomics.ru/jour/article/view/625

Objective: identification of peptides in the composition of Laennec®, which can inhibit the development of endotheliopathy (endothelial dysfunction).Material and methods. Hybrid mass spectrometry followed by data analysis based on topological recognition theory was performed. The analysis of the peptide composition of Laennec® included four stages: purification of the drug, chromatographic separation of peptides, determination of the multidimensional mass spectrum of the peptide fraction, and de novo sequencing of the isolated peptides.Results. The preparation contains peptides-inhibitors of specific target proteins (PRKCZ, PKB, PKD1, MAPK14, IKKB, PDPK1) involved in the activation of the pro-inflammatory transcription factor NF-κB. Inhibition of CDK5 and SHC1 kinases helps to reduce endothelial cell apoptosis. The peptides of the drug also block enzymes involved in the synthesis and maturation of the tumor necrosis factor alpha (MAPKAPK2/3, ADAM17).Conclusion. In the composition of Laennec®, peptides have been found that contribute to a complex pathogenetic action against endotheliopathy. Endothelial regeneration is especially important in the rehabilitation of patients who have recovered from COVID-19.

Цель: выявление пептидов в составе препарата Лаеннек®, которые могут тормозить развитие эндотелиопатии (эндотелиальной дисфункции).Материал и методы. Проведена гибридная масс-спектрометрия с последующим анализом данных на основе топологической теории распознавания. Анализ пептидного состава Лаеннека® включал четыре этапа: очистка препарата, хроматографическое разделение пептидов, определение многомерного масс-спектра пептидной фракции и de novo секвенирование выделенных пептидов.Результаты. В составе препарата идентифицированы пептиды-ингибиторы специфических таргетных белков (PRKCZ, PKB, PKD1, MAPK14, IKKB, PDPK1), вовлеченные в активацию провоспалительного транскрипционного фактора NF-κB. Ингибирование киназ CDK5 и SHC1 способствует снижению апоптоза эндотелиоцитов. Пептиды препарата также блокируют ферменты, участвующие в синтезе и вызревании фактора некроза опухолей альфа (MAPKAPK2/3, ADAM17).Заключение. В составе препарата Лаеннек® найдены пептиды, которые способствуют комплексному патогенетическому действию против эндотелиопатии. Регенерация эндотелия особенно актуальна в реабилитации пациентов, переболевших COVID-19.

дисфункция эндотелияпротеомикакоронавирусная инфекцияполипептидная терапия

endothelial dysfunctionproteomicscoronavirus infectionpolypeptide therapy

Статья подготовлена при поддержке компании «Рхана».

This work was financially supported by the Rhana company.

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The authors declare that there are no conflicts of interest present.