farmaecFARMAKOEKONOMIKA. Modern Pharmacoeconomics and PharmacoepidemiologyФАРМАКОЭКОНОМИКА. Современная фармакоэкономика и фармакоэпидемиология2070-49092070-4933IRBIS LLC10.17749/2070-4909/farmakoekonomika.2024.218farmaec-1093Research ArticleORIGINAL ARTICLESОРИГИНАЛЬНЫЕ ПУБЛИКАЦИИThe influence of the combination of methanolic extract Scurrula atropurpurea (Blume) and Dendrophthoe pentandra on rat liver function and structureВоздействие комбинации метанольного экстракта Scurrula atropurpurea (Blume) и Dendrophthoe pentandra на функцию и структуру печени у крысhttps://orcid.org/0000-0001-5725-0295АтирохН.AthirohN.

Нур Атирох

Джалан Майен Харионо, д.193, Динойо, Лоуквару, Маланг, Восточная Ява 65144

Scopus Author ID: 56106983200

Nour Athiroh

193 Jalan Mayjen Haryono, Dinoyo, Lowokwaru, Malang, Jawa Timur 65144

Scopus Author ID: 56106983200

nour.athiroh@unisma.ac.idhttps://orcid.org/0000-0002-5510-9842МубаракатиН. Дж.MubarakatiN. J.

Нурул Джадид Мубаракати

комплекс Государственного университета Сурабаи D1, Сурабая, Восточная Ява, 60231

Scopus Author ID: 57034189300

 

Nurul Jadid Mubarakati 

Jalan, Komplek Universitas Negeri Surabaya Gedung D1, Surabaya, Jawa Timur, 60231

Scopus Author ID: 57034189300

https://orcid.org/0000-0002-2435-4976ПурномоЮ.PurnomoYu.

Юди Пурномо

Джалан Майен Харионо, д.193, Динойо, Лоуквару, Маланг, Восточная Ява 65144

Scopus Author ID: 57217503005

Yudi Purnomo

193 Jalan Mayjen Haryono, Dinoyo, Lowokwaru, Malang, Jawa Timur 65144

Scopus Author ID: 57217503005

Исламский университет МалангаИндонезияUniversitas Islam MalangIndonesiaГосударственный Университет СурабаиИндонезияUniversitas Negeri SurabayaIndonesia202405112024173337344Copyright © Athiroh N., Mubarakati N.J., Purnomo Y., 20242024Атирох Н., Мубаракати Н.Д., Пурномо Ю.Athiroh N., Mubarakati N.J., Purnomo Y.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.pharmacoeconomics.ru/jour/article/view/1093Background

Background. Tea mistletoe (Scurrula atropurpurea (Blume)) and mango mistletoe (Dendrophthoe pentandra) have been known as a potential medicine for several diseases.

Objective

Objective: to investigate the effect of the combination of methanolic extract Scurrula atropurpurea (Blume) and Dendrophthoe pentandra (MESA-DP) on rat liver function and structure using serological and histopathological analysis.

Material and methods

Material and methods. This study was experimental during 28 days using 20 rats divided into four groups (Group 1 as a control, while Groups 2, 3, and 4 were given MESA-DP at doses 250, 500, and 1,000 mg/kg of body weight, respectively). The liver histopathological structure was observed using hematoxylin-eosin staining. The liver function assessment included total bilirubin, serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), total protein, albumin, globulin, cholesterol, triglycerides, high-density lipoprotein (HDL), and low-density lipoprotein (LDL) levels. Data were analyzed using a one-way ANOVA analysis (ANalysis Of VAriance) and performed via SPSS Statistics 17.0 (IBM, USA).

Results

Results. The administration of MESA-DP did not show significant differences at all doses for the liver rat function in total bilirubin, SGOT, SGPT, albumin, cholesterol and triglycerides (p>0.05), while globulin, total protein, HDL and LDL showed significant results (p<0.05). The liver histopathological structure showed the number of pyknotic, karyorrhectic and karyolytic cells in rats after MESA-DP administration compared to controls, which grew with increasing dose.

Conclusion

Conclusion. The liver function in rats after being exposed to MESA-DP was not affected in terms of total bilirubin, SGOT, SGPT, albumin, cholesterol, and triglyceride levels. However, using MESA-DP increased the necrotic liver cells. It may be beneficial for the liver health of experimental animals taking into account the correct dosage.

Актуальность

Актуальность. Омела чайная (Scurrula atropurpurea (Blume)) и омела манго (Dendrophthoe pentandra) известны как потенциальные лекарственные средства от ряда заболеваний.

Цель

Цель: изучить эффект комбинации метанольного экстракта Scurrula atropurpurea (Blume) и Dendrophthoe pentandra (MESA-DP) на функцию и структуру печени у крыс с помощью серологического и гистопатологического анализа.

Материал и методы

Материал и методы. Исследование проводилось в течение 28 дней с использованием 20 крыс, разделенных на четыре группы: 1-я группа служила контролем, а крысы во 2-й, 3-й и 4-й группах получали MESA-DP в дозах 250, 500 и 1000 мг/кг массы тела соответственно. Гистопатологическую структуру печени наблюдали с помощью окрашивания гематоксилином и эозином. Функцию печени оценивали по сывороточным уровням общего билирубина, глутамат-щавелевоуксусной трансаминазы (ГЩТ), глутамин-пировиноградной трансаминазы (ГПТ), общего белка, альбумина, глобулина, холестерина, триглицеридов, липопротеинов высокой плотности (ЛПВП) и липопротеинов низкой плотности (ЛПНП). Данные проанализированы с использованием однофакторного анализа ANOVA (англ. ANalysis Of VAriance) и программы SPSS Statistics 17.0 (IBM, США).

Результаты

Результаты. Введение MESA-DP при всех дозах не привело к достоверным различиям в функции печени у крыс по показателям общего билирубина, ГЩТ, ГПТ, альбумина, холестерина и триглицеридов (p>0,05), в то время как уровни глобулина, общего белка, ЛПВП и ЛПНП показали значимые различия (р<0,05). Изучение гистопатологической структуры печени продемонстрировало повышенное количество пикнотических, кариоректических и кариолитических клеток у крыс, получавших MESA-DP, по сравнению с контролем, которое росло с увеличением дозы.

Заключение

Заключение. Функция печени после воздействия MESA-DP у крыс не изменилась по показателям общего билирубина, ГЩТ, ГПТ, альбумина, холестерина и триглицеридов. Однако применение MESA-DP привело к увеличению количества некротических клеток печени. Таким образом, оно может оказывать положительный эффект на печень подопытных животных с учетом правильного дозирования.

Dendrophthoe pentandraScurrula atropurpurea (Blume)гистопатологияметанольный экстрактпеченьRattus norvegicusсерологическое исследованиеDendrophthoe pentandraScurrula atropurpurea (Blume)histopathologymethanolic extractliverRattus norvegicusserological analysisReferencesLim Y.C., Rajabalaya R., Lee S.H., et al. Parasitic mistletoes of the Genera Scurrula and Viscum: from bench to bedside. Molecules. 2016; 21 (8): 1048. https://doi.org/10.3390/molecules21081048.Lim Y.C., Rajabalaya R., Lee S.H., et al. Parasitic mistletoes of the Genera Scurrula and Viscum: from bench to bedside. Molecules. 2016; 21 (8): 1048. https://doi.org/10.3390/molecules21081048.Strader D.B., Navarro V.J., Seeff L.B. Chapter 26 – Hepatotoxicity of herbal preparations. In: Boyer T.D., Manns M.P., Sanyal A.J. (Eds.) Zakim and Boyer's hepatology (6th ed.). Philadelphia, PA: Saunders-Elsevier; 2012: 462–75.Strader D.B., Navarro V.J., Seeff L.B. Chapter 26 – Hepatotoxicity of herbal preparations. In: Boyer T.D., Manns M.P., Sanyal A.J. (Eds.) Zakim and Boyer's hepatology (6th ed.). Philadelphia, PA: Saunders-Elsevier; 2012: 462–75.Athiroh N., Permatasari N. Mechanism of action of tea parasite on blood vessels. Jurnal Kedokteran Brawijaya. 2012; 27: 1–4 (in Indonesian).Athiroh N., Permatasari N. Mechanism of action of tea parasite on blood vessels. Jurnal Kedokteran Brawijaya. 2012; 27: 1–4 (in Indonesian).Athiroh N., Sulistyowati E. Scurrula atropurpurea increases nitric oxide and decreases malondialdehyde in hypertensive. Universa Medicina. 2013; 32 (1): 44–50.Athiroh N., Sulistyowati E. Scurrula atropurpurea increases nitric oxide and decreases malondialdehyde in hypertensive. Universa Medicina. 2013; 32 (1): 44–50.Athiroh N., Permatasari N., Sargowo D., Widodo M. Effect of deoxycorticostreone acetate, DOCA; salt to endothelial progenitor cell and cirulating endothelial cell number in rats animal model. In: Proceeding of International Seminar and Symposium of Vacular and Neurodegenerative Diseases, Brawijaya University, Malang. 2013: 16–20.Athiroh N., Permatasari N., Sargowo D., Widodo M. Effect of deoxycorticostreone acetate, DOCA; salt to endothelial progenitor cell and cirulating endothelial cell number in rats animal model. In: Proceeding of International Seminar and Symposium of Vacular and Neurodegenerative Diseases, Brawijaya University, Malang. 2013: 16–20.Athiroh N., Permatasari N., Sargowo D., Widodo M. Antioxidative and blood pressure-lowering effects of Scurrula atropurpurea on deoxycorticosterone acetate-salt hypertensive rats. Biomark Genom Med. 2014; 6 (1): 32–6.Athiroh N., Permatasari N., Sargowo D., Widodo M. Antioxidative and blood pressure-lowering effects of Scurrula atropurpurea on deoxycorticosterone acetate-salt hypertensive rats. Biomark Genom Med. 2014; 6 (1): 32–6.Athiroh N., Sulistyowati E. Evaluation of methanolic extract of Scurrula Atropurpurea (Bl.) dans sub-chronic exposure on Wistar rat liver. Adv Environ Biol. 2015; 9 (23): 245–50.Athiroh N., Sulistyowati E. Evaluation of methanolic extract of Scurrula Atropurpurea (Bl.) dans sub-chronic exposure on Wistar rat liver. Adv Environ Biol. 2015; 9 (23): 245–50.Athiroh N., Wahyuningsih D. Study of superoxide dismutase and malondialdehyde concentrations in mice after administration of methanolic extract of Scurrula atropurpurea (Bl.). Jurnal Kedokteran Hewan – Indonesia. 2017; 11 (1): 19–22.Athiroh N., Wahyuningsih D. Study of superoxide dismutase and malondialdehyde concentrations in mice after administration of methanolic extract of Scurrula atropurpurea (Bl.). Jurnal Kedokteran Hewan – Indonesia. 2017; 11 (1): 19–22.Anjani M., Athiroh N., Mubarakati N. 28-day subchronic study: toxicity test of methanolic extract combination of Scurulla atropurpurea and Dendrophthoe pentandra against renal function impairment of female Wistar rats. Jurnal Ilmiah Biosaintropis (Bioscience-Tropic). 2021; 6 (2): 58–63 (in Indonesian).Anjani M., Athiroh N., Mubarakati N. 28-day subchronic study: toxicity test of methanolic extract combination of Scurulla atropurpurea and Dendrophthoe pentandra against renal function impairment of female Wistar rats. Jurnal Ilmiah Biosaintropis (Bioscience-Tropic). 2021; 6 (2): 58–63 (in Indonesian).Athiroh N., Permatasari N., Sargowo D., Widodo M.A. Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats. Iran J Basic Med Sci. 2014; 17 (8): 622–5.Athiroh N., Permatasari N., Sargowo D., Widodo M.A. Effect of Scurrula atropurpurea on nitric oxide, endothelial damage, and endothelial progenitor cells of DOCA-salt hypertensive rats. Iran J Basic Med Sci. 2014; 17 (8): 622–5.Endharti A., Sulastri E., Umanailo E., et al. Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression. J Appl Pharm Sci. 2018; 8 (7): 10–5. https://doi.org/10.7324/JAPS.2018.8702.Endharti A., Sulastri E., Umanailo E., et al. Mango mistletoe Dendrophthoe pentandra leaf extract acts synergistically with 5-Fluorouracil to induce apoptosis and increase p21 expression in human cervical adenocarcinoma HeLa cells by reducing survivin expression. J Appl Pharm Sci. 2018; 8 (7): 10–5. https://doi.org/10.7324/JAPS.2018.8702.Endharti A.T., Permana S. Extract from mango mistletoes Dendrophthoe pentandra ameliorates TNBS-induced colitis by regulating CD4+ T cells in mesenteric lymph nodes. BMC Complement Altern Med. 2017; 17 (1): 468. https://doi.org/10.1186/s12906-017-1973-z.Endharti A.T., Permana S. Extract from mango mistletoes Dendrophthoe pentandra ameliorates TNBS-induced colitis by regulating CD4+ T cells in mesenteric lymph nodes. BMC Complement Altern Med. 2017; 17 (1): 468. https://doi.org/10.1186/s12906-017-1973-z.Yee L., Fauzi N., Najihah N., et al. Study of Dendrophthoe pentandra ethyl acetate extract as potential anticancer candidate on safety and toxicity aspects. J Anal Pharm Res. 2017; 6 (1): 00167. https://doi.org/10.15406/japlr.2017.05.00167.Yee L., Fauzi N., Najihah N., et al. Study of Dendrophthoe pentandra ethyl acetate extract as potential anticancer candidate on safety and toxicity aspects. J Anal Pharm Res. 2017; 6 (1): 00167. https://doi.org/10.15406/japlr.2017.05.00167.Hasan M., Ali M.T., Khan R., et al. Hepatoprotective, antihyperglycemic and antidiabetic effects of Dendrophthoe pentandra leaf extract in rats. Clin Phytosci. 2018; 4: 16. https://doi.org/10.1186/s40816-018-0076-9.Hasan M., Ali M.T., Khan R., et al. Hepatoprotective, antihyperglycemic and antidiabetic effects of Dendrophthoe pentandra leaf extract in rats. Clin Phytosci. 2018; 4: 16. https://doi.org/10.1186/s40816-018-0076-9.Szurpnicka A., Kowalczuk A., Szterk A. Biological activity of mistletoe: in vitro and in vivo studies and mechanisms of action. Arch Pharm Res. 2020; 43 (6): 593–629. https://doi.org/10.1007/s12272-020-01247-w.Szurpnicka A., Kowalczuk A., Szterk A. Biological activity of mistletoe: in vitro and in vivo studies and mechanisms of action. Arch Pharm Res. 2020; 43 (6): 593–629. https://doi.org/10.1007/s12272-020-01247-w.Lailani M., Edward Z., Herman R.B. Blood pressure of male and female Wistar rats after high salt diet. Jurnal Kesehatan Andalas. 2013; 2 (3): 146–50 (in Indonesian).Lailani M., Edward Z., Herman R.B. Blood pressure of male and female Wistar rats after high salt diet. Jurnal Kesehatan Andalas. 2013; 2 (3): 146–50 (in Indonesian).Arifah S.N., Atho’illah M.F., Lukiati B., Lestari S.R. Herbal medicine from single clove garlic oil extract ameliorates hepatic steatosis and oxidative status in high fat diet mice. Malays J Med Sci. 2020; 27 (1): 46–56. https://doi.org/10.21315/mjms2020.27.1.5.Arifah S.N., Atho’illah M.F., Lukiati B., Lestari S.R. Herbal medicine from single clove garlic oil extract ameliorates hepatic steatosis and oxidative status in high fat diet mice. Malays J Med Sci. 2020; 27 (1): 46–56. https://doi.org/10.21315/mjms2020.27.1.5.Sun S., Wang Y., Wu A., et al. Influence factors of the pharmacokinetics of herbal resourced compounds in clinical practice. Evid Based Complement Alternat Med 2019; 2019: 1983780. https://doi.org/10.1155/2019/1983780.Sun S., Wang Y., Wu A., et al. Influence factors of the pharmacokinetics of herbal resourced compounds in clinical practice. Evid Based Complement Alternat Med 2019; 2019: 1983780. https://doi.org/10.1155/2019/1983780.Hikmah U., Santoso H., Athiroh N. Test of serum glutamic oxaloacetic transaminase levels in wistar rats (Rattus novergicus) after administration of methanolic extract of parasite tea (Scurrula atropurpurea Bl Dans) for 28 days (subchronic exposure). Jurnal Ilmu Biosaintropis. 2016; 2 (in Indonesian).Hikmah U., Santoso H., Athiroh N. Test of serum glutamic oxaloacetic transaminase levels in wistar rats (Rattus novergicus) after administration of methanolic extract of parasite tea (Scurrula atropurpurea Bl Dans) for 28 days (subchronic exposure). Jurnal Ilmu Biosaintropis. 2016; 2 (in Indonesian).Sharma A., Nagalli S. Chronic liver disease. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan.Sharma A., Nagalli S. Chronic liver disease. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 Jan.Panche A.N., Diwan A.D., Chandra S.R. Flavonoids: an overview. J Nutr Sci. 2016; 5: e47. https://doi.org/10.1017/jns.2016.41.Panche A.N., Diwan A.D., Chandra S.R. Flavonoids: an overview. J Nutr Sci. 2016; 5: e47. https://doi.org/10.1017/jns.2016.41.Kurutas E.B. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr Jl. 2015; 15 (1): 71. https://doi.org/10.1186/s12937-016-0186-5.Kurutas E.B. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: current state. Nutr Jl. 2015; 15 (1): 71. https://doi.org/10.1186/s12937-016-0186-5.Minich D.M., Brown B.I. A review of dietary (phyto)nutrients for glutathione support. Nutrients. 2019; 11 (9): 2073. https://doi.org/10.3390/nu11092073.Minich D.M., Brown B.I. A review of dietary (phyto)nutrients for glutathione support. Nutrients. 2019; 11 (9): 2073. https://doi.org/10.3390/nu11092073.Riyadi P.H., Atho'illah M.F., Tanod W.A., Rahmawati I.S. Tilapia viscera hydrolysate extract alleviates oxidative stress and renal damage in deoxycorticosterone acetate-salt-induced hypertension rats. Vet World. 2020; 13 (11): 2477–83. https://doi.org/10.14202/vetworld.2020.2477-2483.Riyadi P.H., Atho'illah M.F., Tanod W.A., Rahmawati I.S. Tilapia viscera hydrolysate extract alleviates oxidative stress and renal damage in deoxycorticosterone acetate-salt-induced hypertension rats. Vet World. 2020; 13 (11): 2477–83. https://doi.org/10.14202/vetworld.2020.2477-2483.Zachary J.F. Pathologic basis of veterinary disease. 6th ed. Mosby; 2016: 1480 pp.Zachary J.F. Pathologic basis of veterinary disease. 6th ed. Mosby; 2016: 1480 pp.López-Vidaña E.C., Pilatowsky F.I., Cortés F.B., et al. Effect of temperature on antioxidant capacity during drying process of mortiño (Vaccinium meridionale Swartz). Int J Food Props. 2017; 20: 294–305. https://doi.org/10.1080/10942912.2016.1155601.López-Vidaña E.C., Pilatowsky F.I., Cortés F.B., et al. Effect of temperature on antioxidant capacity during drying process of mortiño (Vaccinium meridionale Swartz). Int J Food Props. 2017; 20: 294–305. https://doi.org/10.1080/10942912.2016.1155601.Christina Y.I., Nafisah W., Atho’illah M.F., et al. Anti-breast cancer potential activity of Phaleria macrocarpa (Scheff.) Boerl. leaf extract through in silico studies. J Pharm Pharmacog Res. 2021; 9 (6): 824–45.Christina Y.I., Nafisah W., Atho’illah M.F., et al. Anti-breast cancer potential activity of Phaleria macrocarpa (Scheff.) Boerl. leaf extract through in silico studies. J Pharm Pharmacog Res. 2021; 9 (6): 824–45.Wuryandari M.R.E., Widodo N., Widjajanto E., et al. Red Moringa oleifera leaf fermentation extract protecting hepatotoxicity in Balb/C mice injected with Salmonella typhi through Nrf-2, HO-1, and SOD-2 signaling pathways. Res J Pharm Technol. 2020; 13 (12): 5947–52. https://doi.org/10.5958/0974-360X.2020.01038.0.Wuryandari M.R.E., Widodo N., Widjajanto E., et al. Red Moringa oleifera leaf fermentation extract protecting hepatotoxicity in Balb/C mice injected with Salmonella typhi through Nrf-2, HO-1, and SOD-2 signaling pathways. Res J Pharm Technol. 2020; 13 (12): 5947–52. https://doi.org/10.5958/0974-360X.2020.01038.0.

The authors declare that there are no conflicts of interest present.